Part:BBa_K5384007:Design

pPIC9K-His-DDDDK-Vg

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1121
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1121
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 142
  Illegal XhoI site found at 1342
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1121
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1121
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

It is assembled from ordinary plasmids.This composite part contains three His-tag tags (BBa_K5384006), the Vglycin gene (BBa_K5384001), the Enterokinase recognition site (BBa_K5384002), the Asp-pro acid-sensitive site (BBa_K5384003), and upstream the AOX1 promoter (BBa_K5384004), and α-secretion signal peptide (BBa_K5384005).

Source

After inserting the VGLYCIN-encoding polypeptide gene from the Picot yeast plasmid, the VGLYCIN gene and the DDDDK site were ligated to the plasmid pPIC9K.

References

1.WANG Wenli, WANG Yunlong, LI Chenyang, et al. Preparation, Identification and Preliminary Application of His-tagged Monoclonal Antibody[J]. J Cell and Molecular Immunol,2008,24(4):399-400. DOI:10.3321/j.issn:1007-8738.2008.04.028.

2.LI Shuying, ZHAO Zhonglin, NIE Ying, et al. Research Progress on Nattokinase[J]. China Agricultural Science and Technology Review,2013,15(4):139-143.] DOI:10.3969/j.issn.1008-0864.2013.04.21.

3.Huang Zhili, Luo Lixin, Yang Rude, et al. Nattokinase[J]. Chemistry of Life,2000(2):82-83.] DOI:10.3969/j.issn.1000-1336.2000.02.012.